Methodology and resources

Transgenic mouse models, conditional knock-out, segment-specific invalidation
Immortalized cell lines and primary cell culture systems developed from micro-dissected nephron segments
Manipulation of gene expression in cell lines and primary cultures
Promoter analysis (in silico, in vitro)
In situ hybridization, advanced quantitative RT-PCR
Immunoblotting, immunoprecipitation, and immunohisto-/cyto-chemistry
Intracellular distribution studies: subcellular fractionation, immunogold, biotinylation
Ontogeny of transporters and regulators
Transport studies in cells and native tissues (Ussing chamber)
Mouse phenotyping: metabolic cages, special diets, pharmacology interventions
Multisystemic phenotyping: cardiovascular, osmoregulation & thirst
Comprehensive biochemical profiling on dedicated platform optimalized for rodent samples
Development and automation of ELISA
Water and solute transport in mouse model of peritoneal dialysis
Biobanking (all human): end-stage kidney samples (200+); kidney biopsies (500+); urine samples from isolated populations (N=6000); peritoneal biopsies (100+)
DNA cohorts: ADPKD (N=300); rare inherited kidney disorders (N=200); renal transplant (N=300); peritoneal dialysis (N=800)
High throughput analysis of human plasma and urine samples - GWAS
Shared resources through EU-funded EUNEFRON, Genecure, EUROSPAN and EURenOmics consortia

Department of Physiology